ROYAL MONACO RIVIERA WEB MAGAZINE
PAGINA WEB AMBASCIATA D'ITALIA NEL PRINCIPATO DI MONACO
MEDICINE & CHINESE TRADITIONAL MEDICINE
Luigi Mattera is a certified by CERFPA (St. Laurent du Var-France) in HOMEOPATHY & ZUO TUINA MASSAGE OF TRADITIONAL CHINESE MEDICINE - Online certificate from TEXAS CHIROPRACTIC COLLEGE (Pasadena-Texas 2007) in CHIROPRACTIC SPORTS & CHIROPRACTIC TREATMENT OF GOLF INJURIES
HPV AND HUMAN REPRODUCTION
F Borruto, University of Verona, Verona, ITALY - PRINCESSE GRACE HOSPITAL OF MONACO (CHPG)
C Comparetto, Azienda USL 4, Prato, ITALY
Prof Franco Borruto speach at the 27th International Papillomavirus Conference and Clinical Workshop of Berlin on 22nd of September 2011
BACKGROUND: Human reproduction is an inefficient process with losses believed to range from 60% to 80% of fertilizations. Viral infections are likely the second most common cause of spontaneous abortions. Viral agents associated with pregnancy loss include: Herpes Simplex, Rubella, Epstein Barr, and human papillomavirus (HPV).
OBJECTIVES: A number of studies have shown that cervical HPV infection in pregnant women as compared to non pregnant women has a relatively high frequency, that HPVs are elevated in spontaneously aborted products of conception, and that HPVs may be etiologic agents of at least some spontaneous abortions.
METHODS: We conducted apoptosis and invasion assays using extravillous trophoblast cells that were transfected with a plasmid containing the entire HPV16 genome.
RESULTS: Rates of apoptosis were 3- to 6-fold greater in transfected cells than in non-transfected cells or cells transfected with an empty plasmid. Invasion of transfected cells through extracellular matrices was 25-58% lower that of the controls. HPV was detected more frequently in placentas from spontaneous preterm deliveries than in placentas from controls (p = 0.03). Identification of HPV in placentas from cases of pre-eclampsia was not significantly different to controls. HPV infection of extravillous trophoblast induces cell death and may reduce placental invasion into the uterine wall.
CONCLUSION: Our study demonstrated embryo stage-specific effects of HPV on early development. This study suggests the need for increased vigilance to detect HPV infection during early pregnancy, particularly when accompanied by a history of embryonic loss. Immunologic conditions that reduce the likelihood of spontaneous HPV clearance also may limit the likelihood of embryo implantation. Both HPV clearance and intrauterine implantation require activation of a proinflammatory immune response. An innate or acquired decrease in the ability to generate production of high levels of proinflammatory cytokines would explain the current observations.
Declaration of interest