A combination extract of ginseng, epimedium, polygala, and tuber curcumae increases synaptophysin expression in APPV717I transgenic mice

 

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Luigi Mattera is a certified by CERFPA (St. Laurent du Var-France) in HOMEOPATHY (biennale) & ZUO TUINA MASSAGE OF TRADITIONAL CHINESE MEDICINE - Online certificate from TEXAS CHIROPRACTIC COLLEGE (Pasadena-Texas 2007) in CHIROPRACTIC SPORTS & CHIROPRACTIC TREATMENT OF GOLF INJURIES.

In the past, he has been Captain aboard tanker ships . He got  Italian UNIVERSITY DOCTORATE in Foreigner and Litterature Languages (IULM Milano - Italy) and 3 years UNIVERISTY DIPLOMA in Public Relations and Discipline Administration (ISTITUTO UNIVERSITARIO LINGUE MODERNE -  Milano)

PRESENTS:  Royal Monaco Médecine

A combination extract of ginseng, epimedium, polygala, and tuber curcumae increases synaptophysin expression in APPV717I transgenic mice

Jing Shi, Jinzhou Tian, Xuekai Zhang, Mingqing Wei, Long Yin, Pengwen Wang and Yongyan Wang

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Chinese Medicine 2012, 7:13 doi:10.1186/1749-8546-7-13

Published: 9 June 2012

Abstract (provisional)

Background

The density of presynaptic markers of synaptic communication and plasticity, especially synaptophysin (SYP), is significantly correlated with cognitive decline and the progression of Alzheimer's disease (AD), indicating that synaptic protection is an important therapeutic strategy for AD. This study aims to investigate the synaptic protective effects of a combination of several active components extracted from the Chinese herbs ginseng, epimedium, polygala and tuber curcumae (GEPT), in the brains of APPV717I transgenic mice.

Methods

Three-month-old APPV717I mice were arbitrarily divided into 10 groups (n = 12 per group): APP groups receiving vehicle treatment for four or eight months (model groups), three dose groups of GEPT-treated mice for each treatment period, and donepezil-treated mice for each treatment period. Three-month-old C57BL/6J mice (n = 12) were also given vehicle for four or eight months (control groups). Vehicle, donepezil or GEPT were intragastrically administered. Immunohistochemistry (IHC) and Western blot analysis were used to assess protein expression in the hippocampal CA1 region and ratios of SYP to beta-actin levels in hippocampal tissue homogenate, respectively.

Results

Both IHC and Western blot revealed a decrease in SYP levels in the CA1 region of 7- and 11-month-old APPV717I transgenic mice compared with the control groups, whereas SYP levels were increased in donepezil- and GEPT-treated transgenic mice compared with the APP group. There was a significant difference in the levels of SYP detected by IHC between the GEPT high-dose group and the APP group after 4 months of treatment, and there were significant differences between all three GEPT groups and the APP group after 8 months of treatment. Western blotting showed that the SYP protein-beta-actin ratio was decreased in APP mice, while donepezil- and GEPT-treated transgenic mice showed increased trends in the SYP protein-beta-actin ratios.

Conclusion

GEPT increases SYP expression and protects synapses before and after the formation of amyloid plaques in the brains of APPV717I transgenic mice.

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